Assuntos
Hanseníase Virchowiana/diagnóstico , Hanseníase Virchowiana/tratamento farmacológico , Nocardiose/diagnóstico por imagem , Nocardiose/tratamento farmacológico , Infecções Oportunistas/tratamento farmacológico , Corticosteroides/administração & dosagem , Adulto , Azatioprina/administração & dosagem , Biópsia por Agulha , Clofazimina/uso terapêutico , Quimioterapia Combinada , Seguimentos , Humanos , Hospedeiro Imunocomprometido/imunologia , Imuno-Histoquímica , Hanseníase Virchowiana/complicações , Hanseníase Virchowiana/imunologia , Masculino , Nocardiose/complicações , Nocardiose/imunologia , Infecções Oportunistas/diagnóstico , Medição de Risco , Índice de Gravidade de Doença , Tomografia Computadorizada por Raios X/métodos , Resultado do Tratamento , Combinação Trimetoprima e Sulfametoxazol/uso terapêuticoRESUMO
A crude extract from N. brasiliensis cells grown in brain heart infusion culture was analyzed. It showed a complex mixture of at least 37 bands when resolved with the discontinuous buffer system of Laemmli in a gradient SDS-PAGE. Western blot analysis of 16 sera from N. brasiliensis-infected individuals always showed the recognition of six bands of 61, 49, 45, 42, 26, and 24 kilodaltons (kDa). Some other bands also reacted but with less intensity. Sera from tuberculosis and leprosy patients reacted strongly with the 49, 45, and 42 kDa bands but weakly or not at all with the 61, 26, and 24 kDa. Sera from healthy control volunteers reacted with some bands but little or not at all with those three identified by the sera from mycetoma patients. These three immunodominant antigens (61, 26 and 24 kDa) may be of clinical value in the serodiagnosis of mycetoma by N. brasiliensis.
Assuntos
Anticorpos Antibacterianos/biossíntese , Antígenos de Bactérias/imunologia , Micetoma/imunologia , Nocardiose/imunologia , Nocardia/imunologia , Animais , Anticorpos Antibacterianos/sangue , Western Blotting , Eletroforese em Gel de Poliacrilamida , Feminino , Humanos , Soros Imunes/imunologia , Hanseníase/imunologia , Masculino , Coelhos , Tuberculose/imunologiaAssuntos
Feminino , Masculino , Animais , Humanos , Coelhos , Anticorpos Antibacterianos/classificação , Anticorpos Antibacterianos/sangue , Antígenos de Bactérias/imunologia , Eletroforese em Gel de Poliacrilamida , Hanseníase/imunologia , Micetoma/imunologia , Nocardia/imunologia , Nocardiose/imunologia , Soros Imunes/imunologia , Tuberculose/imunologia , Western BlottingRESUMO
A Western blot (immunoblot) assay is presented for the diagnosis of nocardiosis with a specific immunodominant 54-kilodalton (kDa) antigen purified from a culture filtrate of Nocardia asteroides by immunoaffinity chromatography. The chromatography column was prepared with immunoglobulin G obtained from sera from patients with lepromatous leprosy. Unbound solutes consisted of specific, partially purified N. asteroides antigens, primarily a 54-kDa band, accompanied by two others of 31 and 62 kDa. The Western blot technique was applied to detecting the immunologic response to nocardiae. Immunodetection was performed according to the biotin-avidin system, which greatly improved the detection of antibodies even in immunosuppressed hosts. Each of 16 serum samples from immunosuppressed or immunocompetent patients infected with N. asteroides reacted with the 54-kDa band, and two reacted with the 31- and 62-kDa bands. Each of the serum specimens obtained from patients with mycetoma caused by Nocardia brasiliensis or Rhodococcus rhodochrous reacted with the 54-kDa band. There was no reaction to either the 54- or the 31-kDa antigen with all serum samples obtained from patients with tuberculosis, except one, with all serum samples obtained from patients with leprosy, or with all sera obtained from healthy controls. The 54-kDa protein is a candidate to be used as a probe to study the humoral immunologic response to nocardiae.
Assuntos
Antígenos de Bactérias/isolamento & purificação , Western Blotting/métodos , Nocardiose/diagnóstico , Nocardia asteroides/imunologia , Anticorpos Antibacterianos/análise , Cromatografia de Afinidade , Reações Cruzadas , Humanos , Peso Molecular , Nocardiose/imunologiaRESUMO
Sera from 23 patients with nocardiosis were tested for the presence of precipitating antibodies against an extract of Nocardia asteroides. Sera from nine of 20 patients infected with Nocardia asteroides and one of three sera from patients with Nocardia brasiliensis produced one to three precipitin lines with the antigen. Sera from eight of 55 patients with tuberculosis and 11 of 13 patients with leprosy also contained Nocardia precipitins. None of 33 sera from patients with cryptococcosis, blastomycosis, actinomycosis, histoplasmosis, coccidioidomycosis, mucormycosis or candidiasis contained Norcardia antibodies. None of the sera from ten patients infected with Staphylococcus aureus, ten patients infected with Pseudomonas, or 16 sera from uninfected patients contained Nocardia precipitins.